安徽农业科学2011,Vol.39Issue(34):20992-20993,2.
忍冬属植物茎的同工酶谱分析研究
Study on Peroxidase Isozyme of Stem of Lonicera L.
摘要
Abstract
[Objective] The aim was to study Peroxidase (POD) isozyme of Lonkera L. For providing theoretical basis for their variety identification. [ Method] Vertical polyacrylamide gel electrophoresis (PAGE) was used to determine the Peroxidase (POD) isozyme of stem of four species of Lonicera L. And one specie of Kolkwitzia Grarbn. [ Result ] There are 7 bands of POD isozymes in five materials and POD-1 and POD-2 were the two bands which appear in all of the materials. POD-6 only appeared in four materials of Lonicera L. And POD-3 and POD-7 only appeared in K. Amabilis Grarbn. So it could be seen as the marker of Lonicera L. Clustering analysis showed that all of the plants could be divided into 2 groups, in which L. Tragophylla Hemsl,L. Nervosa Maxim,L. Chrysantha Turcz. ,and L. Caendea Linn, belonged to the first group,and K. Amabilis Grarbn belonged to the second group. [Conclusion] POD isozyme analysis has certain application value in classification and identification of Lonicera L.关键词
忍冬属/茎/POD同工酶Key words
Lonicera L. / Stem/ POD Isozyme分类
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马玉花,伊万梅,沈宁东,宋维秀,杨春江,安胜虎..忍冬属植物茎的同工酶谱分析研究[J].安徽农业科学,2011,39(34):20992-20993,2.基金项目
青海大学博士科研启动项目(2008-QGC-8). (2008-QGC-8)