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1株猪链球菌的分离·鉴定及其毒力因子研究

江南屏 任瑶瑶 戴甑 蓝小玲 李学如 姚宁

安徽农业科学2011,Vol.39Issue(34):21034-21036,3.
安徽农业科学2011,Vol.39Issue(34):21034-21036,3.

1株猪链球菌的分离·鉴定及其毒力因子研究

Identification of a Strain of Streptococcus suis and the Main Virulence Factors Analysis

江南屏 1任瑶瑶 1戴甑 1蓝小玲 1李学如 1姚宁1

作者信息

  • 1. 西南交通大学生命科学与工程学院,四川成都 610031
  • 折叠

摘要

Abstract

[Objective] To identify a Streptococcus strain, so as to provide theoretical basis for further studying the toxicity of Streptococcus suis. [ Method ] The identification of the strain was performed by Gram stain and microscopy, and 16S rDNA gene sequence analysis. Seven main virulence genes were selected to design primers and the virulence gene distribution was detected by PCR. [Result] The strain was Gram positive bacterium, presenting chain - like distribution. The 16S rDNA sequence of the strain was above 99% of homology to Streptococcus suis 2. [Conclusion] The isolated strain was Streptococcus suis 2. The virulence genotype of this SS2 was: gdh+/ef+/mrp+/sly+/jbps+/orf2+/cps2J+/.

关键词

猪链球菌/分离/毒力因子/16S rDNA

Key words

Streptococcus suis/ Isolation/ Virulence factors/ 16S rDNA

分类

农业科技

引用本文复制引用

江南屏,任瑶瑶,戴甑,蓝小玲,李学如,姚宁..1株猪链球菌的分离·鉴定及其毒力因子研究[J].安徽农业科学,2011,39(34):21034-21036,3.

基金项目

中央高校基本科研业务费专项资金资助(SWJTU09BR226). (SWJTU09BR226)

安徽农业科学

0517-6611

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