安徽农业科学2011，Vol.39Issue(34)：21072-21073,21076,3.
压砂甜瓜白粉病病原菌18S rDNA序列分析
Analysis of 18S rDNA Sequence of Melon Powdery Mildew from Lands Overlaid with Sands in Ningxia
摘要
Abstract
[Objective] The aim was to analyze the 18S rDNA sequence of melon powdery mildew from lands overlaid with sands. [Method] Thepathogen of melon powdery mildew was isolated from infected plants of "Yujinxiang" ,a major melon variety eultivated in lands overlaid with sandsof droughty midregion in Ningxia. Genome DNA was extracted from its conidia using Chelex-100 method. 18S rDNA sequence was amplified byPCR,which was analyzed by Blast after sequencing,and the phylogenetic tree was constructed. [ Result] 18S rDNA sequence analysis showed thatthe pathogen of melon powdery mildew belonged to Podosphaera. [Conclusion] The study provided reference for biocontrol and disease-resistancebreeding against melon powdery mildew.关键词
甜瓜白粉病/18S rDNA/分类鉴定Key words
Melon powdery mildew /18S rDNA /Taxonomic identification分类
农业科技引用本文复制引用
杨静玲,刘建利..压砂甜瓜白粉病病原菌18S rDNA序列分析[J].安徽农业科学,2011,39(34):21072-21073,21076,3.基金项目
宁夏回族自治区自然科学基金项目(NZ0958). （NZ0958）
