安徽农业科学2011，Vol.39Issue(34)：21323-21324,21327,3.
HPLC-ELSD法快速检测白茶等3种茶叶中L-茶氨酸的含量
Rapid Detection of L-theanine in Three Kinds of Tea with HPLC-ELSD
王汉领 1宗素艳 2陆斌 2万红贵1
作者信息
- 1. 南京工业大学生物与制药工程学院,江苏南京210009
- 2. 南京同凯兆丰生物科技有限公司,江苏南京210009
- 折叠
摘要
Abstract
[Objective] To provide reliable detection methods for extracting and preparing L-theanine. [Method] High Performance Liquid Chromatography-Evaporative Light Scattering Detection (HPLC-ELSD) was used to quantitatively detect the L-theanine content in white tea, Biluochun and Cuizhu. [Result] On the C18 column in reversed phase column,with 0. 15% trifluoroacetic acid (containing 0.06% heptafluorobutyric acid) in aqueous solution-acetonitrile (88:12) as mobile phase,L-theanine could be effectively detected. The detection limit concentrations of L-theanine was 0. 2 - 1.0 mg/ml,the corresponding concentration ( Y) and peak area (X) were linear related, the linear equation was Y = 2 × 10-7AT +0.005 7,the correlation coefficient was 0.999 6,the average recoveries of L-theanine was 99.55% to 100% and relative standard deviation was 0.39% - 1.00% . [Conclusion] The method was precise .accurate, highly stable, simple, convenient and rapid for the quantitative determination of L-theanine form the extracted liquid of teas.关键词
HPLC-ELSD/七氟丁酸/L-茶氨酸/茶叶Key words
HPLC-ELSD/ Heptafluorobutyric acid/ L-theanine/ Tea分类
农业科技引用本文复制引用
王汉领,宗素艳,陆斌,万红贵..HPLC-ELSD法快速检测白茶等3种茶叶中L-茶氨酸的含量[J].安徽农业科学,2011,39(34):21323-21324,21327,3.
