安徽农业科学2011，Vol.39Issue(35)：21577-21579,3.
海石竹叶片的离体培养与植株再生
Culture in vitro and Plant Regeneration of Armeria maritima Leaves
摘要
Abstract
[Objective] To investigate culture in vitro and plant regeneration of Armeria maritima leaves. [ Method] With a basal medium MS, we made research into the callus induction, cluster buds and root differentiation on the tips, central and base of Armeria maritima leaves respectively. [Result] As to the callus induction, using leaves tip as the explants received the best effect, followed by the leaves central, and leaves base was the worst. The optimal medium for callus induction from leaves tip was MS +6-BA 2.0 mg/L + NAA 0.75 mg/L and the optimal medium for callus induction from leaves central and base was MS + 6-BA 4. 0 mg/L + NAA 0. 25 mg/L. The optimal medium for cluster buds was MS +6-BA 3.0 mg/L + NAA 0.10 mg/L. The optimal medium for root differentiation was MS + NAA 0.20 mg/L. [ Conclusion] The high-efficiency in vitro culture and rapid regeneration system was established, which laid foundation for the scale and industrialized production and future scientific research of Armeria maritima.关键词
海石竹/激素配比/离体培养/植株再生Key words
Armeria maritima/ Hormone combination/ Culture in vitro/ Plant regeneration分类
农业科技引用本文复制引用
吴仕雅,刘帆,臧薇,李方安..海石竹叶片的离体培养与植株再生[J].安徽农业科学,2011,39(35):21577-21579,3.基金项目
四川农业大学本科论文培养计划项目(2010-1278). （2010-1278）
