安徽农业科学2011，Vol.39Issue(35)：21582-21583,2.
拟南芥两个MYB基因标签融合蛋白转基因植株的获得和鉴定
Formation of the Transgenic Arabidopsis Plant with Two MYB Genes Tagging the Fusion Protein and Its Identification
邹明学 1申宇欢 2陈艳红2
作者信息
- 1. 南通大学公共卫生学院,江苏南通226019
- 2. 南通大学生命科学学院,江苏南通226019
- 折叠
摘要
Abstract
[Objective] The downstream genes regulated by the MYB gene: At3g11280 and At5g05790, were developed through the experiment in chromosome immunoprecipitation(ChIP). [Method] Based on the construction of the plant expression vectors tagging fusion protein, the transgenic Arabidopsis plants were produced after these vectors were introduced into the plants by means of the agrobacterium-mediated transformation, which expression in transgenic plants was detected by Western blotting method. [ Result] The transgenic efficiency of Arabidopsis plants was very high and more than 20 transgenic plants were produced under the transformation of four-tagged protein fusion vectors. The Western blotting analysis result from four random 8 Tl plants of four types of transgenic plants showed that the positive plants could be founded in the four types of transgenic plants. The band of fusion protein was about 30 Kd and it can be seen that the size of displaying band was consistent with that of predicted protein after the band with color signal was compared with the Marker. [Conclusion] These transgenic plants expressing fusion protein would be the important material for ChIP experiment.关键词
MYB基因/拟南芥转化/转基因植株鉴定Key words
MYB gene/ Arabidopsis transformation/ Identification of transgenic plant分类
农业科技引用本文复制引用
邹明学,申宇欢,陈艳红..拟南芥两个MYB基因标签融合蛋白转基因植株的获得和鉴定[J].安徽农业科学,2011,39(35):21582-21583,2.
