安徽农业科学2011,Vol.39Issue(35):21670-21673,4.
马齿苋中抗氧化物质的超声提取工艺优选
Study on Optimization of Ultrasonic-assisted Extraction of Antioxidants from Portulaca oleracea L.
摘要
Abstract
[Objective] To study the optimum extraction technology of antioxidants from Portulaca oleracea L. [ Method] On the basis of single-factor experiment,the effects of ethanol concentration,liquid-solid ratio,extraction time and extract times on scavenging rate of DPPH radical were inspected. According to the experimental design principles of Box-Benhnken,a four-factor and three-level response surface modeling (RSM) was adopted to study the ultrasonic extraction technology of antioxidant from Portulaca oleracea L. [ Result] Based on analyzing the significance of each factor and their interactions,the optimum extraction conditions were determined as follows:the ethanol concentration was 68% ,the solid-liquid ratio was 1:22, the extraction time was 44 min and the extraction times was 3. Under the optimized conditions, the actual scavenging rate of DPPH free radical was 0.911 7,close to the predicted value. [Conclusion]The ultrasonic method had the advantages of high extraction rate,short time and low ultrasonic power,therefore it could be the method for extracting antioxidant compounds from Portulaca oleracea L.关键词
马齿苋(Portulaca oleracea L.)/抗氧化/超声波提取法/DPPH 自由基/响应面分析法Key words
Portulaca oleracea L. / Antioxidantion/ Ultrasonic-assisted extraction/ DPPH free radical/ Response surface methodology分类
医药卫生引用本文复制引用
陆娟,刘春明,杨帆,成宝海,李新玲..马齿苋中抗氧化物质的超声提取工艺优选[J].安徽农业科学,2011,39(35):21670-21673,4.基金项目
吉林省教育厅项目(吉教科合字2010379) (吉教科合字2010379)
长春师范学院资助项目. ()