安徽农业科学2011，Vol.39Issue(35)：21755-21756,21759,3.
固定化漆酶酶促合成茶黄素工艺的研究
Study on Technology of Synthesizing Theaflavins through Enzyme-catalysing Oxidation with Immobilized Laccase
摘要
Abstract
[Objective ] The study aimed to explore the technology of synthesizing the theaflavins through the enzyme-catalysing oxidation with the immobilized laccase. [Method] The theaflavins was synthesized from tea polyphenol through in vitro enzyme-catalysing oxidation with D152 resin immobilized laccase. The effects of the reaction temperature, pH value, enzyme concn. , reaction time and speed of adding oxygen on synthesizing the theaflavins was studied though the single factor experiment, on base of which, the synthesizing reaction through the enzyme-catalysing oxidation was conducted on the theaflavins and then the yield of the theaflavins was determined. [Result] The immobilized laccase was got by the immobilization with the resin carrier D152,the enzyme-catalysing activity by the immobilized laccase was about 70% of the original catalytic activity after the repetition use of the resin for 5 times and that still retained 48% or so after the repetition use for 10 times. The best conditions for the enzyme-catalysing oxidation with the immobilized laccase were as followsjthe reaction temperature of 60 ℃ ,best pH value of 5.0,substrate concn. Of 2.0 g/L,adding oxygen speed of 20 L/min and reaction time of 1.5 h. [Conclusion] Using the resin carrier D152 could effectively fix the laccase. Compared with the method of the enzyme-catalysing oxidation with the free laccase, the immobilized method could effectively increase the stability and utilization efficiency of the laccase and its reaction products also could be easy for the purification.关键词
固定化漆酶/酶促氧化/茶多酚/茶黄素Key words
Immobilized laccase/ Enzymatic oxidation/ Tea polyphenol/ Theaflavins分类
农业科技引用本文复制引用
岳鹍,揣玉多,孙勇民..固定化漆酶酶促合成茶黄素工艺的研究[J].安徽农业科学,2011,39(35):21755-21756,21759,3.
