安徽农业科学2011，Vol.39Issue(36)：22217-22219,22276,4.
胡桃EST-SSR标记的筛选及引物设计
Development of Walnut EST-SSR Markers and Primer Design
摘要
Abstract
[Objective] This research aimed to develop walnut EST-SSR markers and design corresponding primers. [Method] 5213 EST sequences of walnut (Juglans regia Linn. ) public online in NCBI were used for character analysis with bioinformatics methods, and primers were designed for the selected EST sequences by using Primer 3.0 software. [ Result] 207 SSRs were obtained from the EST sequences, including 188 non-redundant sequences, the detection rate was 3. 97% with an average distribution distance of 21. 12 kb. Totally 92 types of repeat motifs were involved, which were mainly composed of dinucleotide and trinucleotide, accounting for 31.40% and 35.27% of the total number of repeat motifs, respectively. 30 pairs of primers were initially selected from the 50 randomly-selected SSR primers by PCR amplification. [ Conclusion ] This research would lay foundations for the development of EST-SSR molecular markers in walnut and design of the targeted EST-SSR primers by mining and analyzing the SSR sites in walnut EST sequences.关键词
胡桃/EST-SSR/微卫星/引物Key words
Walnut/ EST-SSR/ Microsatellite/ Primers分类
农业科技引用本文复制引用
冯怡,张智俊,张舍龙,罗淑萍..胡桃EST-SSR标记的筛选及引物设计[J].安徽农业科学,2011,39(36):22217-22219,22276,4.基金项目
浙江省自然科学基金资助项目(Y307469)和浙江农林大学人才启动基金项目. （Y307469）
