安徽农业科学2011，Vol.39Issue(36)：22564-22565,22570,3.
三甲基氯硅烷涂层毛细管胶束电动色谱法测定苯甲酸和邻苯二甲酸
Determination on Benzoic Acid and Phthalic Acid by Micellar Electrokinetic Chromatography with Trimethylchlorosilane-coated Capillaries
摘要
Abstract
[ Objective] The study aimed to establish the new method for determining the contents of the benzoic acid and phthalic acid by the micellar electrokinetic chromatography with the coated capillaries. [Method] The new method of micellar electrokinetic chromatography by u-sing the trimethylchlorosilane coated capillaries with UV diode array detection was used for the separation and determination of benzoic acid in the drink and benzoic acid and phthalic acid in the laboratory waste water. The effects of the wavelength, pH value, buffer solution, voltage and other factors on the separation and determination of the benzoic acid and phthalic acid were investigated. [ Result ] Under the optimized operation conditions of 20 mmol/L SDS + 35 mmol/L borate(pH of9.5)as the running buffer solution, the detection wavelength of 210 nm, and the separation voltage of 14 kV, the benzoic acid and phthalic acid could be separated in 3 min, their good linear relationships between the peak heights and concn. Were obtained in the range of 2 -800 μg/L, with their correlation coefficients being 0.999 3 and 0.999 9 resp, the RSD of migration time being 2. 65% and 3. 69% resp. , the RSD of peak height being 3. 12% and 4. 52% , resp. , and the detection limits being 0.1067 and 0. 121 3 μg/ml resp. [ Conclusion] This new measuring method had the characters of less sample dosage, easy operation and good sensitivity and accuracy, which provided an effective method for the analysis of the preservatives.关键词
胶束电动色谱/涂层/苯甲酸/邻苯二甲酸/三甲基氯硅烷Key words
Micellar Electrokinetic Chromatography ( MEKC) / Coating/ Benzoic acid/ Phthalic acid/ Trimethylchlorosilane分类
轻工纺织引用本文复制引用
谢建平,任凯,刘中奎,刘秋艳,邓洪瑞,王晓璐,张亚楠,翟海蝶..三甲基氯硅烷涂层毛细管胶束电动色谱法测定苯甲酸和邻苯二甲酸[J].安徽农业科学,2011,39(36):22564-22565,22570,3.基金项目
河南省高校青年骨干教师资助计划项目(2004162) （2004162）
周口师范学院大学生科研创新基金项目(zknudxs201146) （zknudxs201146）
周口师范学院实验室开放项目(201109). （201109）
