安徽农业科学2012，Vol.40Issue(1)：36-38,40,4.
藻蓝蛋白α亚基裂合酶的分子克隆和酶动力学研究
Study on Molecular Cloning and Enzyme Kinetics of Anabaena α-Phycocyanin Lyase
摘要
Abstract
To comparatively study the differences on structures and functions of phycocyanin lyase CpcE/F from different cyanobacteria, CpcE/F from Anabaena sp. PCC 7120 were cloned and expressed greatly. The reconstitution of PCB and CpcA from Mastigocladus laminosus PCC 7603 in vitro using the overexpressed lyase Anabaena sp. PCC 7120 CpcE/F showed that CpcE/F from Anabaena sp. PCC 7120 is the specific enzyme for biosynthesis of α-PC, furthermore, the enzyme kinetics of PcE/F lyase were studied preliminarily.关键词
鱼腥藻/藻蓝蛋白/CpcE/CpcF/重组/酶动力学Key words
Anabaena/ Phycocyanin/ CpcE lyase/ CpcF lyase/ Reconstitution/ Enzyme kinetics分类
农业科技引用本文复制引用
苏平,周明..藻蓝蛋白α亚基裂合酶的分子克隆和酶动力学研究[J].安徽农业科学,2012,40(1):36-38,40,4.基金项目
湖北省教育厅科学技术研究资助项目(D20092201) （D20092201）
湖北师范学院人才引进项目(2008F16)和高效博士学科专项科研基金批准项目(20060487018). （2008F16）
