安徽农业科学2012，Vol.40Issue(1)：451-453,3.
固相萃取-高效液相色谱法测定城市污水中痕量多环芳烃的研究
Study on the Determination of Trace Amount of Polycyclic Aromatic Hydrocarbons in Urban Sewage by Solid-phase Extraction Coupled with High Performance Liquid Chromatograph
摘要
Abstract
[ Method ] The study aimed at determining trace amount of polycyclic aromatic hydrocarbons ( PAHs) in urban sewage by using solid-phase extraction (SPE) coupled with high performance liquid chromatograph (HPLC). [Method] From the aspects of solid-phase extraction column, elution solvent, elution volume, elution speed and so forth, the test conditions of SPE-HPLC method were optimized, and trace amounts of PAHs in urban sewage was determined by this method. [Result] The optimized solid-phase extraction conditions were SUPEL-CLEAN LC-18 solid-phase extraction column, methylene dichloride as elution solvent, 15 ml elution volume, 2 ml/min elution speed, 5 ml/min loading speed, 1 000 ml water with 200 ml methanol loading volume. Under the optimized extraction conditions, the average recovery was high, namely 76.3% -105.2% ; relative standard deviation was 3.8% -6.0% , showing good precision; detection limit was low, only 0.000 8-0.048 μg/L. [ Conclusion] The method is user-friendly, with high sensitivity and good precision, and suitable for continuous determination of a large volume of water.关键词
固相萃取/高效液相色谱/多环芳烃/城市污水Key words
Solid-phase extraction (SPE)/ High performance liquid chromatograph ( HPLC ) / Polycyclic aromatic hydrocarbons (PAHs)/Urban sewage分类
资源环境引用本文复制引用
荣楠,王靖飞,康全影,吴亦红,李洪波..固相萃取-高效液相色谱法测定城市污水中痕量多环芳烃的研究[J].安徽农业科学,2012,40(1):451-453,3.基金项目
国家水体污染控制与治理科技重大专项(2012 ZX07209-003). （2012 ZX07209-003）
