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珍贵树种山白兰的组织培养研究

乔梦吉 郑明朝 宋小秋 俞建妹 江蓓 谭菲 黄柏华 蒋中鑫

安徽农业科学2012,Vol.40Issue(2):655-657,3.
安徽农业科学2012,Vol.40Issue(2):655-657,3.

珍贵树种山白兰的组织培养研究

Study on Tissue Culture of Rare Species Paramichelia baillonii

乔梦吉 1郑明朝 2宋小秋 1俞建妹 2江蓓 1谭菲 1黄柏华 1蒋中鑫1

作者信息

  • 1. 广西大学林学院,广西南宁53004
  • 2. 南宁良凤江国家森林公园,广西南宁530031
  • 折叠

摘要

Abstract

[Objective] The aim was to research the rapid reproduction of the tissue culture in Paramichelia baillonii (DC) Hu. [ Method] Taking the stems with buds from the potted seedlings of P. Baillonii as the explants and MS medium as the basic medium, the tissue culture test of P. Baillonii was carried out through the addition of the plant growth regulators with different types and concn. In the medium. [ Result ] The best but induction medium was MS +0.5mg/L6 -BA +0. Lmg/LNAA, with the but induction rate of 88.9% ; the optimum medium for proliferation was MS+0.3mg/L6-BA +0.05mg/L NAA +1.0mg/L KT, with the bud proliferation coefficient of 2. 6; the rooting induction had a greater difficulty and the medium of 1/2MS +2.0mg/L NAA could get the rooting rate of 54.5%. [Conclusion] The study provided the reference for the further development and application of P. Baillonii and the abundance of Guangxi forest tree germplasm resources.

关键词

珍贵树种/山白兰/组织培养

Key words

Rare species/ Paramichelia baillonii ( DC) Hu/ Tissue culture

分类

农业科技

引用本文复制引用

乔梦吉,郑明朝,宋小秋,俞建妹,江蓓,谭菲,黄柏华,蒋中鑫..珍贵树种山白兰的组织培养研究[J].安徽农业科学,2012,40(2):655-657,3.

基金项目

广西林业科技项目(桂林科字[2009]第22号 ()

广西大学科研基金项目(XJZ110610). (XJZ110610)

安徽农业科学

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