安徽农业科学2012，Vol.40Issue(2)：675-677,3.
番茄幼苗根系总蛋白SDS-PAGE方法的优化
Optimization of SDS-PAGE Procedures for Analyzing the Total Protein In Seedling Roots of Lycopersicon esculentum
摘要
Abstract
[Objective] This study aimed to develop an efficient SDS-PAGE method suitable for analyzing the total protein of Lycopersicon esculentum seedling roots. [ Method] By using the L. Esculentum seedling roots as the experimental material, the protein extraction, sample volume and other factors influencing the final electrophoresis pattern were respectively optimized. [Result] The high-resolution, clear and reproducible SDS-PAGE pattern could be obtained when the protein was extracted with modified TCA-acetone precipitation and the sample volume for e-lectrophoresis was 60 μg. [ Conclusion ] This optimized method could basically meet the requirements for study on the proteome of tomato seedling roots.关键词
番茄根系/蛋白组学/SDS-PAGEKey words
Tomato roots/ Proteome/ SDS-PAGE分类
农业科技引用本文复制引用
邢佳毅,雷慧,程继鸿,杨瑞,王绍辉..番茄幼苗根系总蛋白SDS-PAGE方法的优化[J].安徽农业科学,2012,40(2):675-677,3.基金项目
教育部科学研究重点课题资助(210001). （210001）
