安徽农业科学2012，Vol.40Issue(2)：719-722,4.
鱼腥草叶乙醇提取液的抑菌作用研究
Study on the Antimicrobial Activity of Ethanol Extracts from H.cordata Leaves
摘要
Abstract
[Objective] To study the optimum extraction technology of flavonoids from H. Cordata leaves and the antimicrobial effect of ethanol extracts from H. Cordata leaves. [ Method] With 70% ethanol as solvent, L9(34) orthogonal test was designed to optimize the extraction technology of flavonoids from H. Cordata leaves. Then, the antimicrobial activity of ethanol extracts from H. Cordata leaves against B. Polymyxa, E. Coli, S. Aureus, B. Subtilis, S. Pombe, S. Cerevisiaeand A. Niger was investigated. [Result] The optimum condition for extracting flavonoids with ethanol was based on twice extraction of each 2 h at 80 ℃ with the solid - liquid ratio of 1:20 g/ml. Under the above condition, the extraction rate of flavonoids from H. Cordata leaves was 4.363%. Ethanol extracts of H. Cordata leaves had broad - spectrum antibacterial activity, which was more effective for bacteria than fungus. The minimum inhibitory concentration of H. Cordata leaves extracts against B. Polymyxa, E. Coli, S. Aureus, B. Subtilis, S. Pombe, S. Cerevisiae and A. Niger was 0.06, 0.06, 0.08, 0.08, 0.1, 0.1 and 0. 1 g/ml, respectively. [ Conclusion] This study could provide theoretical basis for the further development and utilization of H. Cordata /eaves.关键词
鱼腥草(Houttuynia cordata Thunb.)/提取物/最小抑菌浓度Key words
H. Cordata Thunb. /Extracts/ Minimum inhibitory concentration分类
农业科技引用本文复制引用
黄亮,王金龙,李方,童应凯,王玉..鱼腥草叶乙醇提取液的抑菌作用研究[J].安徽农业科学,2012,40(2):719-722,4.基金项目
国家自然科学基金资助项目(30900191). （30900191）
