安徽农业科学2012，Vol.40Issue(2)：729-730,2.
不同产地金银花与山银花中绿原酸含量的比较研究
Comparative Study on the Content of Chlorogenic Acid in LONICERAE JAPONICAE FLOS and LONICERAE FLOS from Different Producing Areas
摘要
Abstract
[Objective] To compare the differences in the content of chlorogenic acid between Shandong Pingyi, Henan Fengqiu LONICERAE JAPONICAE FLOS and Hunan Longhui, Chongqing Xiushan, Chongqing Wulong, Chongqing Jiangjin LONICERAE FLOS. [Method] The content of chlorogenic acid in LONICERAE JAPONICAE FLOS and LONICERAE FLOS from different producing areas was determined by RP-HPLC. The separation was performed on a chromatographic column Platisil ODS (250 mm×4.6 mm, 5 μm) with the detection wavelength at 326 nm. Following the injection of sample solution, an elution with acetonitrile-0.2% phosphoric acid as mobile phase (17: 83,K/K) was initiated at a flow rate of 1.0 ml/min. [Result] The content of chlorogenic acid in Hunan Longhui, Chongqing Xiushan, Chongqing Wulong and Chongqing Jiangjin LONICERAE FLOS was 9. 03% , 8. 62% , 4. 51% and 3. 71% respectively, and that in Shandong Pingyi and Henan Fengqiu LONICERAE JAPONICAE FLOS was 4. 08% and 3. 79%. Of them, the content of chlorogenic acid was the highest in Chongqing Xiushan LONICERAE FLOS. [Conclusion] This method is reliable, stable and sensitive with high precision, strong resolving power and high recovery rate, and could be used for determining the content of chlorogenic acid in different producing areas of LONICERAE JAPONICAE FLOS and LONICERAE FLOS.关键词
绿原酸/金银花(LONICERAE JAPONICAE FLOS)/山银花(LONICERAE FLOS)反相高效液相色谱法/含量Key words
Chlorogenic acid/ LONICERAE JAPONICAE FLOS/ LONICERAE FLOS/ RP-HPLC/ Content分类
医药卫生引用本文复制引用
冯彬彬,王小翠,张建海,徐晓玉..不同产地金银花与山银花中绿原酸含量的比较研究[J].安徽农业科学,2012,40(2):729-730,2.基金项目
重庆市"十一五"规划项目 （）
秀山县政府资助. （）
