安徽农业科学2012，Vol.40Issue(2)：748-751,4.
大孔吸附树脂分离纯化桂花总黄酮工艺研究
Study on the Isolation and Purification Process of Total Flavonoids from Osmanthus fragrans Lour.with Macroporous Absorption Resin
摘要
Abstract
[Objective] To study the isolation and purification process of total flavonoids from Osmanthus fragrans Lour. With macroporous absorption resin. [Method] Taking the adsorption capacity and recovery of total flavonoids as index, the optimum the isolation and purification process of total flavonoids from Guizhou O. Fragrans were respectively investigated by static test and dynamic test with AB-8 macroporous absorption resin. [Result] pH, eluant, temperature, concentration of column-loading sample, diameter-height ratio, current velocity and quality ratio of total flavonoids and absorption resin had great effects on the adsorption capacity and recovery of total flavonoids from O. Fragrans. The optimum conditions for extracting and purifying total flavonoids in static test were; the AB-8 type macroporous resin was blended with 4 times of pH 4-5sample solution (solid-liquid ratio of 1 :4 g/ml) together and then stirred to make it reach the saturation adsorption; then, the isolated resin whose volume was 9.4 times as high as total flavonoids in sample solution was eluted with 70% ethanol. The optimum conditions in dynamic test were: 17. 86 mg/ml the extracts of total flavonoids from O. Fragrans were loaded into the column with diameter-height ratio of 1. 5 :21. 6 at a current velocity of 2 - 3 ml/min and then eluted with 2-3 BV of water to remove impurity at a current velocity of 3 - 4 ml/min. As the temperature increased, the adsorption capacity declined but the elution rate to enlarge. [ Conclusion] The optimum conditions for extracting and purifying total flavonoids with AB-8 type macroporous resin are selected in this study, which could provide experimental basis for the industrial production of total flavonoids from O. Fragrans.关键词
桂花(Osmanthus fragrans Lour.)/总黄酮/分离/纯化/大孔吸附树脂/工艺条件Key words
Osmanthus fragrans Lour. / Total flavonoids/ Isolation/ Purification/ Macroporous resin/ Technological conditions分类
医药卫生引用本文复制引用
郁建生,罗显华..大孔吸附树脂分离纯化桂花总黄酮工艺研究[J].安徽农业科学,2012,40(2):748-751,4.基金项目
贵州省高层次人才科研条件特助经费项目:TZJF-2007-64号 （）
贵州省省地科技合作项目黔科合区域合[ 2009] 7012-1号 （）
贵州省科技创新人才团队建设资助项目:黔科合人才团队[2010]4008号. （）
