安徽农业科学2012，Vol.40Issue(2)：761-762,2.
柳叶中黄酮醇苷元槲皮素的含量测定
Determination of Quercitin in Willow Leaf
刘卫 1邓淑凤 1刘景东 1秦葵 1孙晓丽1
作者信息
- 1. 第四军医大学白求恩军医学院,河北石家庄050081
- 折叠
摘要
Abstract
[Objective] The aim was to develop a method of RP-HPLC for determination of flavnoid aglycones - quercitin in willow leaf,and to discover its content. [Method] Willow leaves were collected in the middle of April. The flavonoids in the leaves were extracted by successive extraction with methanol. The extracts were acid-hydrolyzed before being tested with RP-HPLC. The condition of RP-HPLC was: Diamonsil C18 column (150 mm×4.6 mm,5 μm); concentration of mobile phase was 0.07% ; phosphoric acid-water-free ethanol (50:50,V/V); mobile speed was 1.0 ml/min; tested wave length 260 nm; sampling amount 10 μl; flexibility 0.000 1 AUFS; column temperature was indoor temperature (20 ℃). [ Result] The constituent of flavnoid aglycones in willow leaves was mainly quercitin and it consisted of 0.36% -0.74% flavnoid aglycones. [Conclusion] The method of RP-HPLC for determination of quercitin in willow leaf provided reference for the effective use of willow.关键词
柳叶/槲皮素/高效液相测定Key words
Quercitin/ Willow leaf/ RP-HPLC分类
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刘卫,邓淑凤,刘景东,秦葵,孙晓丽..柳叶中黄酮醇苷元槲皮素的含量测定[J].安徽农业科学,2012,40(2):761-762,2.
