安徽农业科学2012，Vol.40Issue(2)：780-781,785,3.
微波辅助提取黎豆种皮黑色素
Extraction of Melanin from the Seed Coat of Mucuna prurients ( L.) by Microwave-assisted Method
董华群 1汪跃华 2董华强 2高赛文2
作者信息
- 1. 贵阳护理职业学院,贵阳市金阳新区石林路550081
- 2. 佛山大学食品系,广东佛山市南海区大沥528231
- 折叠
摘要
Abstract
[Objective] To research on the extraction technology of melanin from the seed coat of Mucuna prurients (L. ) by microwave-assisted alkaline extraction and acid precipitation. [ Method] Melanin was extracted from the seed coat of M. Prurients by the method of microwave-assisted alkaline extraction-acid precipitation and the method of alkaline extraction-acid precipitation. Content of melanin in M. Prurients was detected by UV spectrophotometry. [ Result] The melanin content of the seed coat was 2.41%. The optimal conditions for alkaline extraction-acid precipitation were 1.0 mol/L NaOH solution, 1:20 (g/ml) solid-liquid ratio,4 -6 h extraction time,and pH =2 for the precipitation of melanin. Under these conditions, the extraction rate of the crude extracts of melanin was (95.3 ±1.5)%; and the purity of melanin reached (37.4 ±1.5)%. [ Conclusion ] The seed coat of M. Prurients was rich in melanin, which deserved further research and development. Microwave assistance could significantly enhance the extraction rate of the crude extracts of melanin and greatly shortened extraction time remarkably.关键词
黎豆/黑色素/微波/提取Key words
Mucuna prurients (L. ) / Melanin/ Microwave/ Extraction分类
轻工纺织引用本文复制引用
董华群,汪跃华,董华强,高赛文..微波辅助提取黎豆种皮黑色素[J].安徽农业科学,2012,40(2):780-781,785,3.
