安徽农业科学2012,Vol.40Issue(2):791,895,2.
厚朴叶多糖铁复合物中铁的含量测定
Determination of Fe(Ⅲ) from the Polysaccharide -Fe(Ⅲ) Complex of CORTEX MAGNOLIAE OFFICINALIS Leaves
摘要
Abstract
[Objective] To determine the Fe(Ⅲ) content in polysaccharide - Fe(Ⅲ) complex of CORTEX MAGNOLIAE OFFICINALIS leaves. [ Method ] Polysaccharide was extracted from the leaves of CORTEX MAGNOLIAE OFFICINALIS. Under alkaline condition, polysaccharide - Fe ( Ⅲ) complex was formed. And the content of Fe(Ⅲ) was determined by UV spectrophotometry. [ Result] when the Fe(Ⅲ) content was between 0 and 0.196 7 mg/ml,absorbance and concentration showed good linear relationship (r =0.999 8); the average recovery rate was 98. 83% and RSD was 1.60% ; Fe(Ⅲ) content in polysaccharide - Fe(Ⅲ) complex of CORTEX MAGNOLIAE OFFICINAIIS leaves was 31.42%. [ Conclusion] This method was rapid and reliable and could be used for the Fe(Ⅲ) content in polysaccharide - Fe( Ⅲ) complex of CORTEX MAGNOLIAE OFFICINALIS leaves.关键词
厚朴叶多糖铁(Ⅲ)复合物/铁含量/分光光度法Key words
Polysaccharide-Fe(Ⅲ) complex/ CORTEX MAGNOLIAE OFFICINALIS leaves/ Fe(Ⅲ) content/ Spectrophotometry分类
农业科技引用本文复制引用
邓芬,张芳芹,穆琳,盛维娟,刘存芳,赖普辉..厚朴叶多糖铁复合物中铁的含量测定[J].安徽农业科学,2012,40(2):791,895,2.基金项目
陕西理工学院2011年大学生创新试验项目(UIRP1131). (UIRP1131)