安徽农业科学2012，Vol.40Issue(3)：1300-1303,4.
毛果杨1-羟基-2-甲基-2-(E)-丁烯基-4-二磷酸合酶(HDS)基因的生物信息学分析
Bioinformatics Analysis of 4-hydroxy-3-methyl-2- buten1yl diphosphate synthase (HDS) in Populus trichocarpa
刘立辉 1李成浩 1杨静莉 1夏德安1
作者信息
- 1. 东北林业大学林木遗传育种国家重点实验室,黑龙江哈尔滨150040
- 折叠
摘要
Abstract
[ Objective] The aim was to study the homology and evolutionary relationship among HAD genes. [ Method] Based on HDS gene of Populus trichocarpa, bioinformatics software and website were used to predict and analyze base distribution, amino acids composition, hydrophil-city or hydrophobicity, hyperconservative region, secondary structure, and three-level structure. The results were used to compare with HDS gene sequences of other ten species and related evolution analysis was followed. [ Result ] mRNA, single chain of HDS gene of Populus trichocarpa , was 1 956 bp in length, code was made up of proteins containing amino acids of 656, and relative molecular mass of protein was 72 937.03 with Leu of 10. 50% involume; in polypeptide, hydrophobic amino acid was only of 39.63% with hydrophilc region of 10 and hy-drophobic region of 6; after many comparisons with other ten species, homology was found as high as 99%. [ Conclusion ] HDS gene of Populus trichocarpa was quite stable and coded albumen was hydrophilic protein which was quite conservative in evolution; sequence information in conservative region gained in the test paved the way for clone of novel gene.关键词
HDS/毛果杨/生物信息学/萜类Key words
HDS/ Populus trichocarpa/ Bioinformatics information/ Hemiterpen分类
生物科学引用本文复制引用
刘立辉,李成浩,杨静莉,夏德安..毛果杨1-羟基-2-甲基-2-(E)-丁烯基-4-二磷酸合酶(HDS)基因的生物信息学分析[J].安徽农业科学,2012,40(3):1300-1303,4.
