安徽农业科学2012，Vol.40Issue(3)：1304-1306,3.
超声波处理对大白菜花粉管通道法转基因的影响
Influence of Ultrasonic Treatment of Chinese Cabbage Gene Transformation through the Pollen Tube Pathway
摘要
Abstract
[Objective] The Research aimed to explore improving the percent conversion of Chinese cabbage through pollen tube pathway. [ Method] The Chinese cabbage variety: Dabaicai No. 03 and Yuqing being taken as experimental material, the parameters of the germination pollen treated by buffer and ultrasonic was analyzed. [ Results ] The best buffer for cabbage pollen germination was the sucrose of 200 g/L + boric acid of 100 mg/L + calcium nitrate of 200mg/L and the preferred processing parameters was the ultrasonic power of 150w, the working time of 5 seconds, the time interval of 5 seconds, the processing time of 8 seconds. Under the selection concentration of 200 mg/L of Kanamy-cin, 3 Tl plants were obtained. [Conclusion] The experiment laid the basis for further genetic transformation of Chinese cabbage.关键词
超声波/大白菜/花粉管通道法/转基因Key words
Ultrasonic/ Chinese cabbage/ Pollen tube pathway/ Transgenic method分类
农业科技引用本文复制引用
狄红梅,王学府,曹秋芬,孟玉平..超声波处理对大白菜花粉管通道法转基因的影响[J].安徽农业科学,2012,40(3):1304-1306,3.基金项目
山西省科技攻关项目(021034) （021034）
山西省农科院科研项目(YGX-01). （YGX-01）
