安徽农业科学2012，Vol.40Issue(3)：1309-1312,4.
青钱柳愈伤组织诱导与增殖的初步研究
A Preliminary Study on Callus Induction and Proliferation of Cylocarya paliurus
摘要
Abstract
[Objective] To carry out preliminary study on callus induction and proliferation of Cylocarya paliurus. [Method] The leaf and stem segments of Cylocarya paliurus as explants were cultured on MS, WPM and improved DKW3 mediums, added 6-BA and IBA with different concentration ratios to explore the optimum medium for callus induction and proliferation. [ Result] It was found that MS +4.0 mg/L 6-BA + 2.0 mg/L IBA was the optimum medium for inducing stem callus of Cylocarya paliurus, MS +2.0 mg/L 6-BA +0.5 mg/L IBA was the optimum medium for inducing leaf explants callus of Cylocarya paliurus, and MS + 1.0 mg/L 6-BA + 1.0 mg/L IBA was the optimum medium for callus proliferation. 200 mg/L Vitamin C was best to inhibit Cylocarya paliurus callus from browning with the browning rate of only 5. 71%. [ Conclusion ] This study provided some theoretical basis for the establishment of tissue culture and rapid propagation system and the development of molecular breeding study of Cylocarya paliurus.关键词
青钱柳/愈伤组织诱导/增殖/褐化Key words
Cylocarya paliurus/ Callus induction/ Proliferation/ Browning分类
农业科技引用本文复制引用
王纪,谢寅峰,方升佐..青钱柳愈伤组织诱导与增殖的初步研究[J].安徽农业科学,2012,40(3):1309-1312,4.基金项目
国家林业公益性行业科研专项(200904046) （200904046）
江苏高等学校优秀科技创新团队项目(苏教科[2009]10号) （苏教科[2009]10号）
江苏省"六大人才高峰"资助项目. （）
