安徽农业科学2012，Vol.40Issue(3)：1340-1342,1345,4.
基因枪介导的玉米NPR1基因的遗传转化
Genetic Transformation of NPR1 Gene into Maize by Microprojectile Bombardment
摘要
Abstract
[Objective] The aim was to explore the conditions of transformation in maize by microprojectile bombardment. [Method] Immature embryonic callus of maize inbred line 7239 were used as explants to study the effects of shoot distance, helium pressure, vacuum and bombardment frequency on the transformation efficiency in the particle bombardment system of maize. [Result] Considering the transformation efficiency, particle bombardment with 100 μg/P of golden particles, at a shoot distance of 9 cm from the target cells, under helium pressure of 1 350 psi and vacuum 25 In · Hg, and bombarding twice could achieve relatively ideal results. After selection on media supplemented with different concentration of hygromycin, some regenerated plants were obtained. The results of PCR and Southern blotting analysis demonstrated that the NPRl gene had been integrated into the genome of transgenic maize plants, with an average transformation efficiency of 1. 76%. [Conclusion] The study had laid the foundation for the cultivation and breeding of excellent resistant varieties of maize.关键词
玉米/愈伤组织/NPR1基因/基因枪/遗传转化Key words
Maize/ Callus/ NPRl gene/ Particle bombardment/Genetic transformation分类
农业科技引用本文复制引用
秦新民,李惠敏,曾振华,覃屏生,高成伟..基因枪介导的玉米NPR1基因的遗传转化[J].安徽农业科学,2012,40(3):1340-1342,1345,4.基金项目
广西自然科学基金资助项目(桂科自0991096). （桂科自0991096）
