安徽农业科学2012，Vol.40Issue(3)：1343-1345,3.
小菜蛾化学感受蛋白突变体的构建
A Mutant of PlxyCSP1 Protein in Plutella xylostella
摘要
Abstract
[Objective] This study aimed to explore the effects of cysteine (Cys58) on the characteristics of PlxyCSPl in P. Xyostella combined with pesticide compounds. [Method] Cys58 of PlxyCSPl was mutated into Trp58 by using overlap extension PCR method, and PlxyCSPl -M2 mutant was obtained. Expression vector was constructed and the protein was detected by western blot. [Result] Expression vector pET32a- PlxyCSPl -M2 was constructed to express the 35 kDa weight protein. [Conclusion] PlxyCSPl mutant protein was expressed successfully in prokaryotic expression system.关键词
小菜蛾/化学感受蛋白1/原核表达Key words
P. Xylostella/ PlxyCSPl/ Prokaryotic expression system分类
生物科学引用本文复制引用
刘晶晶,胡美英,任珍珍,李亚楠..小菜蛾化学感受蛋白突变体的构建[J].安徽农业科学,2012,40(3):1343-1345,3.基金项目
广东省自然科学基金(9451064201003679) （9451064201003679）
广东省科技项目(2009B020310005). （2009B020310005）
