安徽农业科学2012，Vol.40Issue(3)：1346-1347,1351,3.
microRNA表达水平检测技术的研究进展
Advances in the Detection Technique of microRNA Expression Level
摘要
Abstract
[Objective]The aim was to summarize the research progress of microKINA( miruNA) expression detection technique, to lay the foundation for miRNA expression study and it' s functional control. [Methods] Through summarizing the principles, characteristics and progress of miRNA expression level detection technique, to explain how to detect it' s expression simply and effectively. [Result] miRNA is an important class of small non-protein coding RNA, regulate and control a series of important life processes, it' s expression study is the important approach for the study of it' s control function, the detection method for miRNA expression are Northern blot, In Situ Hybridization, ISH, Expression Profile Chip, RT-PCR and cloning and sequencing. [Conclusion] The study provides methods for detecting miRNA expression simply and effectively, and lay the foundation for expounding it' s control mechanism for important life processes of animals and plants.关键词
microRNA/表达水平/检测技术Key words
microRNA/ Expression level/ Detection technique分类
生物科学引用本文复制引用
黄京书,武华玉,乔木,冯政,刘贵生,梅书棋..microRNA表达水平检测技术的研究进展[J].安徽农业科学,2012,40(3):1346-1347,1351,3.基金项目
国家自然科学基金(30901025) （30901025）
湖北省重点实验室项目(2011ZD123). （2011ZD123）
