安徽农业科学2012，Vol.40Issue(3)：1348-1351,4.
一株地衣芽孢杆菌的性质研究及发酵培养基优化
Research on the Character and Optimization of Fermentation Medium for Bacillus licheniformis, KL6
摘要
Abstract
[Objective] The aim was to study the feasibility of separating Bacillus licheniformis, KL6, as microbial feed additives, optimized its fermentation medium, and determined the optimum culture condition so as to provide a basis of industrial fermentation. [Method] The physiological and biochemical and enzyme-producing of Bacillus licheniformis, KL6, were evaluated by phenotypic tests. Fermentation medium and conditions by and 4- factors and 3-levels orthogonal and single-factor expts improved the culture medium and optimized fermentation condition. [Result] The number of viable cell was influenced by four factors in order of significance as follows:MnSO4 > NaCl > Yeast extract > Peptone, the number of spores was MnSO4 > Peptone > NaCl > Yeast extract, and the optimum culture condition was: Peptone 1% , Yeast extract 0. 5% , NaCl 1% , 25% MnSO4. The suitable temperature of Bacillus licheniformis was 37 °C. The fermentation process of the fermenter should be stop at 14-16h, at that time the number of viable cell was 101.63X109per milliliter, and the rate of spores was 97. 11%. [Conclusion] On the basis of the results of in vitro evaluation test, Bacillus licheniformis, KL6, had potential as microbial feed additives. On the basis of results that optimized fermentation medium and conditions,Bacillus licheniformis, KL6, was suitable for high-density fermentation of liquid. This experiment provided a theoretical basis to the development of microbial feed additives and large-scale fermentation which was based on KL6.关键词
地衣芽孢杆菌/微生物饲料添加剂/发酵/培养基优化/正交试验Key words
Bacillus licheniformis/ Microbial feed additives/ Fermentation/ Medium optimization/ Phenotypic test分类
生物科学引用本文复制引用
刘阳,谭明,潘宝平,宋诙..一株地衣芽孢杆菌的性质研究及发酵培养基优化[J].安徽农业科学,2012,40(3):1348-1351,4.基金项目
天津市科技计划项目(10ZCKFSY06400). （10ZCKFSY06400）
