安徽农业科学2012，Vol.40Issue(3)：1407-1408,1651,3.
高效液相色谱法测定不同年限三七根中皂苷含量
High-performance Liquid Chromatography in Different Years Thirty-seven Saponin Content
摘要
Abstract
[Objective J The content of sapomns in the root of Panax notoginsengy Burk. ) F.. H. Chen with different growth ages was measured.[Method] By means of high-performance liquid chromatography, the main root notoginseng the content of the saponin in the root of Panax notoginseng( Burk. ) F. H. Chen from Wenshan areas of Yunnan was tested and the change in the content of the saponin of the samples with different planting years was explored. The chromatographic condition was: the column was Kromasil ODS Cl8(250 mm ×4.6 mm,5 μm) , the acetonitrile-water as mobile phase was used for gradient elution, the flow rate was 1. Oml/min, the wavelength of UV in detector was 203 ran and the column temperature was 25 ℃.[Results] with the increment of growth age, the content of saponin in root of Panax notoginseng (Burk. ) F. H. Chen was relatively increased and the best planting year was in 3 years to 4 years. [Conclusion] The method was simple and could be used in the determination of saponins.关键词
高效液相色谱/三七(Panax notoginseng(Burk.)F.H.Chen)/皂苷Key words
High performance liquid chromatography/ Panax notoginseng (Burk. ) FHChen/ Saponin分类
医药卫生引用本文复制引用
朱伟伟,王齐..高效液相色谱法测定不同年限三七根中皂苷含量[J].安徽农业科学,2012,40(3):1407-1408,1651,3.基金项目
广西教育厅科研立项项目(201010LX496) （201010LX496）
引进人才科研启动费项目(2008QS-N012) （2008QS-N012）
昆明理工大学分析测试中心主任基金(2008-1). （2008-1）
