安徽农业科学2012，Vol.40Issue(3)：1448,1492,2.
马铃薯中糖苷生物碱的提取研究
Study on Extraction of Glycoalkaloids in Potato
摘要
Abstract
[Objective] The study aimed to optimize the extraction methods and conditions of the glycoalkaloids in potato. [Method] With the fresh potato peel was the tested material, 7 extraction methods including the acetic acid extraction-ammonia precipitation, ultrasonic auxiliary acid extraction-ammonia precipitation, methanol extraction-ammonia precipitation, double solvent extraction, mixed solvent extraction, acid alcohol extraction-ammonia precipitation, and acetic acid and sodium sulfite extraction- ammonia precipitation were used to extract the glycoalkaloids in potato. The effects of the extraction temperature, ultrasonic time and acid concentration on the extraction effect were investigated through the single factor test to optimize the extraction methods and conditions of the glycoalkaloids in potato.[Result] Among the 7 extraction methods, the method of ultrasonic auxiliary acid extraction-ammonia precipitation could get best extraction effect. Among 3 extraction conditions, the extraction temperature of 30 ℃ was suitable, the ultrasonic time of 20 min was suitable and the acidity of 25% was the best. [Conclusion] The extraction method of directly adding the some concn. Of acid combined with the ultrasonic oscillating without using the organic solvent showed the characteristics of simple operation, short extraction cycle and good extraction effect.关键词
马铃薯/糖苷生物碱/提取Key words
Potato/ Glycoalkaloids/ Extraction分类
农业科技引用本文复制引用
薄丽丽,孙晓彦,林浩,戴明婧,牛继平,张金文,王蒂..马铃薯中糖苷生物碱的提取研究[J].安徽农业科学,2012,40(3):1448,1492,2.基金项目
"973"计划前期研究专项(2010CB134404) （2010CB134404）
甘肃省自然科学基金项目(0710RJZA088). （0710RJZA088）
