安徽农业科学2012，Vol.40Issue(3)：1457-1458,2.
顶果木离体培养研究
Study on Tissue Culture of Acrocarpus fraxinifolius
周传明 1秦武明 1吕曼芳 1熊英2
作者信息
- 1. 广西大学林学院,广西南宁5300041
- 2. 广西大学农学院,广西南宁530004
- 折叠
摘要
Abstract
[Objective] The aim of the study was to seek the best method on tissue culture of Acrocarpus fraxinifoliu. [Method] The multi-buds induction and regeneration system of Acrocarpus fraxinifolius from seed in vitro was preliminary investigated. [ Result] The results showed that the MS+6-BA0. 8 -3.0 mg/L + NAA 0. 2-0.5 mg/L culture medium,modified MS+ 6-BA 0.2 - 1.2 mg/L + NAA 0. 18-0.2 mg/L culture medium could maintain seedling growth, which modified MS + 6-BA 0. 2 mg/L + NAA 0.18 mg/L culture medium was the most suitable for seedling growth, but the bud differentiation were not happened after 40 days. The modified MS + 6-BA 0.5 -1.2 mg/L + NAA 0.2 mg/L culture medium could induce bud differentiation and proliferation. The propagation coefficient of the bud could reach 1. 72 -2. 30 after 20 days, which the propagation coefficient was 2. 30 that culture in modified MS +6-BA 1. 2 mg/L + NAA 0. 2 mg/L medium, the overall shoots growth were not significant. [ Conclusion] The modified MS +6-BA 1.2 mg/L + NAA 0.2 mg/L culture medium was the most suitable for tissue culture of Acrocarpus fraxinifoliu.关键词
顶果木/无菌苗/离体培养Key words
Acrocarpus fraxinifolius/ Aseptic seedlings/ In vitro culture分类
农业科技引用本文复制引用
周传明,秦武明,吕曼芳,熊英..顶果木离体培养研究[J].安徽农业科学,2012,40(3):1457-1458,2.
