安徽农业科学2012，Vol.40Issue(4)：1959-1960,2002,3.
番茄同源四倍体离体诱导研究
Study on in Viro Induction of Autotetraploid of Tomato(Lycopersicon esculentum Mlll.)
吴志刚 1刘丹赤 1宋明2
作者信息
- 1. 日照职业技术学院食品工程学院,山东日照276826
- 2. 西南大学园艺园林学院,重庆400716
- 折叠
摘要
Abstract
[Objective] Study on in viro induction of autotetraploid of tomato was conducted by using the conbation of the tissue culture technology and the colchicine treatment. [ Method] With the tomato variety Yufen 109 as the tested material, based on the establishment of the tomato plant efficient in viro regeneration system, taking the cotyledons callus with the green bud growing point as the induction materials, the in viro induction of autotetraploid of tomato was conducted reap. By means of mixed culture method and immersion method with the colchicine. [ Result] When taking the cotyledons callus with the green bud growing point as the induction materials and in viro induction of autotetraploid of tomato was adopted by the mixed culture method, the treatment with 0. 2% colchicines for 48 h could get highest induction rate, being 50% , while that by the immersion method got higher induction rate, but had a great harm on the explant. [ Conclusion ] The higher induction rate was obtained by using the mixed culture method, which provided a new way for the improvement of variety and innovation and abundance of germplasm resources in the tomato.关键词
番茄/组织培养/同源四倍体/诱导Key words
Tomato/Tissue culture/Autotetraploid/Induction分类
农业科技引用本文复制引用
吴志刚,刘丹赤,宋明..番茄同源四倍体离体诱导研究[J].安徽农业科学,2012,40(4):1959-1960,2002,3.
