安徽农业科学2012，Vol.40Issue(4)：1961-1962,2069,3.
苔藓植物matK基因PCR反应体系的建立和优化
Establishment and Optimization of matK gene-PCR Amplification System for Bryophytes
摘要
Abstract
[Objective] To establish and optimize the matK gene-PCR reaction system for Bryophytes. [ Method ] Using genomic DNA of Bryo-phytes extracted via an improved CTAB method, single factor analysis was performed to investigate the impacts of DNA template concentration, primer concentration, 2 x Taq MasterMix concentration on matK gene-PCR amplification and to optimize this system for Bryophytes. [Result] The matK gene-PCR amplification(10.0 μl) suitable for Bryophytes was determined to be composed of 0.5 μl DNA, 0.2 μl primer and 5.6 μl 2 × Taq MasterMix. [ Conclusion] The study laid basis for analyzing the molecular systematics of Bryophytes.关键词
苔藓/matK/优化/反应体系Key words
Bryophytes/ matK/ Optimization/ Reaction system分类
生物科学引用本文复制引用
郑红建,张安世..苔藓植物matK基因PCR反应体系的建立和优化[J].安徽农业科学,2012,40(4):1961-1962,2069,3.基金项目
河南省教育厅自然科学研究计划项目(2010C180004) （2010C180004）
