安徽农业科学2012，Vol.40Issue(4)：2015-2016,2033,3.
马铃薯脱病毒与快繁技术研究
Study on Virus-free and Rapid Propagation Technique of Potato
赵艳 1王海新 1苏君伟 1尤淑丽1
作者信息
- 1. 辽宁省风沙地改良利用研究所,辽宁阜新123000
- 折叠
摘要
Abstract
[Objective] The study aimed to get the virus-free tube plantlets of the potato and rejuvenat the tube plantlets that had the growth potential degradation in the long-term subculture. [ Method ] The different sterilization methods and media were set up to carry out the study on the virus-free and rapid propagation technique of the potato. [ Result] The combinations of 5% NaCIO for 2 min and 0.1% HgCl2 for 3 min could get the optimum sterilization effects on the bud explants of the potato. The optimum medium for the induction of the shoot tip was MS + GA3 0. 1 mg/L + 6-BA 0.5 mg/L + IBA 0.2 mg/L,with the mean induction rate of 58. 25%. Comparison between the 2 media of MS liquid and MS solid showed that MS liquid medium had the action of rejuvenating the tube plantlets. Comparison between the different inoculation patterns of the cutting propagation and the horizontal propagation indicated that the horizontal inoculation pattern could accelerate the reproductive speed of virus-free tube plantlets. [ Conclusion ] The study provided the reference basis for the tissue culture and rapid propagation of the potato.关键词
马铃薯/培养基/灭菌/组织培养Key words
Potato/Medium/Sterilization/Tissue culture分类
农业科技引用本文复制引用
赵艳,王海新,苏君伟,尤淑丽..马铃薯脱病毒与快繁技术研究[J].安徽农业科学,2012,40(4):2015-2016,2033,3.
