安徽农业科学2012，Vol.40Issue(4)：2041-2042,2089,3.
膜超滤分离美洲大蠊抗肿瘤活性部位小分子肽的研究
Separation of Antitumor Active Plypeptides from Periplaneta americana by Ultra Filtration Technology
摘要
Abstract
[Objective] The paper was to establish ultrafiltration membrane technology for separation of antitumor active plypeptides from Periplaneta americana. [ Method] Hollow fiber ultrafiltration membrane(3 kDa) was used to separate antitumor active plypeptides from P. Amer-icana. Through single factor test,the impact of rotation speed,concentration ratio,pH and operation time on separation effort was studied,and the separation technology conditions were further optimized through orthogonal test. [ Result ] The best technology conditions for separation of antitumor active plypeptides from P. Americana were as follows: rotation speed 4500 r/min, concentration ratio 30% ,pH 11, and the rotation speed was the major influencing factor. [ Conclusion] The study laid foundation for industrialization of ultrafiltration membrane separation of antitumor active plypeptides from P. Americana.关键词
超滤/膜污染/蛋白质/美洲大蠊Key words
Ultrafiltration/ Membrane fouling/ Protein/ Periplaneta americana分类
生物科学引用本文复制引用
吴少辉,彭芳,高鹏飞,巫秀美,何苗,张成桂,刘光明..膜超滤分离美洲大蠊抗肿瘤活性部位小分子肽的研究[J].安徽农业科学,2012,40(4):2041-2042,2089,3.基金项目
云南省重点产业创新工程基金项目(2008IF012) （2008IF012）
