安徽农业科学2012，Vol.40Issue(4)：2110,2208,2.
省沽油组培过程中外植体灭菌技术的研究
Research on Explants Sterilization Technique in Staphylea bumalda DC.Tissue Culture Process
摘要
Abstract
[Objective] The aim was to seek appropriate explants sterilization method in Staphyiea bumalda DC. Tissue culture process. [ Method] The comparative method was used to study the effect of alcohol , HgCl2, HgCl2 + alcohol combination in different sterilization time on explants growth. [ Result] The results showed that the appropriate explant sterilization technology was the shoot tips survival rate was 100% with 1% HgCl2+70% alcohol ,processing time was 5 min + 50 s respectively; The stem sections survival rate was 95% with 1% HgCl2,processing time was 15 min; The young leaves survival rate was 85% with 1% HgCl2, processing time was 12 min. [Conclusion] 1% HgCl2 + 70% alcohol, 1% HgCl, was effective sterilant of Staphyiea bumalda DC. Explants.关键词
省沽油/组织培养/外植体/灭菌技术Key words
Staphyiea bumalda DC. /Tissue culture/Explants/ Sterilization分类
农业科技引用本文复制引用
朱秀蕾,毕璋友,饶敏..省沽油组培过程中外植体灭菌技术的研究[J].安徽农业科学,2012,40(4):2110,2208,2.基金项目
安徽省高等学校省级自然科学研究项目(KJ2010B068) （KJ2010B068）
