安徽农业科学Issue(5)：2574-2576,3.
三聚氰胺单克隆抗体制备与鉴定
Preparation and Identification of Anti-Melamine Monoclonal Antibody
摘要
Abstract
[Objective] To prepare the anti-melamine monoclonal antibodies and determinate their titer,sensitivity and specificity. [ Method] The spleen cells of BALB/c mice immunized by MEL-BSA were fused with SP2/0 myeloma cells,and hybridomas secreting monoclonal antibodies a-gainst melamine were selected and cloned. The titer,sensitivity and specificity of the monoclonal antibodies were determined by indirect ELISA method. [Result] Two stable hybridoma cell lines, named 1B12 and 2H9 were established. The titer of the monoclonal antibodies secreted by 1B12 and 2H9 determined by indirect ELISA were 1:25 600 and 1:12 800, respectively. The monoclonal antibodies had a good sensitivity with an ICX of 8.0 ng/ml and 8.3 ng/ml and 100% cross-reactivity to melamine,and scarcely showed cross-reactivity to others. [Conclusion] The anti-melamine special monoclonal antibodies with high titer and sensitivity were obtained, which laid foundation for future research.关键词
三聚氰胺/单克隆抗体Key words
Melamine/ Monoclonal antibody分类
轻工纺织引用本文复制引用
王旻子,应永飞,龚云飞,方结红,陈宗伦,张明洲..三聚氰胺单克隆抗体制备与鉴定[J].安徽农业科学,2012,(5):2574-2576,3.基金项目
浙江省重大科技专项(2009C12055) （2009C12055）
浙江省重点科技创新团队(2010R50028). （2010R50028）
