安徽农业科学Issue(5)：2659-2660,2663,3.
虎杖中白藜芦醇苷不同检测方法的比较
Comparison of Different Methods for the Detection of Polydatin in RHIZOMA ET RADIX POLYGONI CUSPIDATI
摘要
Abstract
[ Objective] To compare the content of polydatin in artificial cultivated RHIZOMA ET RADIX POLYGONI CUSPIDATI determined by different methods. [ Method] High-performance liquid chromatngraphy ( HPLC) and capillary electrophoresis (CE) were adopted to determine the content of polydatin in the artificial cultivars of RHIZOMA ET RADIX POLYGONI CUSPIDATI. Then,the linear range,quantitative reproducibility .detection limit and analysis time of the separation between the two methods were comparatively studied. [ Result J High-performance liquid chromalography with fluorescence detection ( HPLC-KD) was the optimum method for detecting polydatin in RHIZOMA ET RADIX POLYGONI CISPIDATI. Following the injection of 10 μl of sample solution,polydatin was separated on a chromatographie column Diamonsil-C18(250 mm x 0.46 mm,5 μm) with 25% acetonitrile as mobile phase at the column temperature of 25 ℃,and then quantified by fluorescence detection with the excitation wavelength at 334 nm and the emission wavelength at 408 nm. The flow rate of mobile phase was set to 1.0 ml/min. [ Conclusion] HPLC is an ideal method to determine the content of polydatin in RHIZOMA ET RADIX POLYGONI CUSPIDATI.关键词
虎杖(RHIZOMA ET RADIX POLYGONI CUSPIDATI)/白藜芦醇苷/高效液相色谱/毛细管电泳Key words
RHIZOMA ET RADIX POLYGON/CLSPIDATI/ Polydatin/ HPLC/ CE分类
医药卫生引用本文复制引用
陈爽,周国海,皮小武,贺丽萍,曹庸..虎杖中白藜芦醇苷不同检测方法的比较[J].安徽农业科学,2012,(5):2659-2660,2663,3.基金项目
国家林业局[2007]108号 （）
