安徽农业科学Issue(5)：2669-2670,2673,3.
HPLC法测定榛子雄花中槲皮素和山萘素的含量
Determination the Content of Quercetin and Kaempferol in Male Flower of Corylus heterophylla Fisch.
徐建 1王婷婷 1杨波 1张显涛1
作者信息
- 1. 吉林修正药业新药开发有限公司,吉林长春 130012
- 折叠
摘要
Abstract
[Objective] To establish an HPLC method for determining the content of quercetin and kaempferol in male flower of Corylus heterophylla Fisch. [ Method] 4 HPLC analysis was performed on a Diamonsil C18 column (250 mm×4. 6 mm,5 μm) with the detection wavelength at 360 nm. Following the injection of sample solution,a elution with methanol -0.4% phosphoric acid ( V/V,50:50) as mobile phase was initiated at a flow rate of 1.0 ml/min. [ Result ] The quercetin showed a good relationship in the range of 0.022 to 0.658 μg (r =0.999 9). The average recovery of quercetin was 99. 4% with the RSD of 0. 73%. The kaempferol showed a good relationship in the range of 0.012 1 to 0. 362 0 μg (r =0. 999 9). The average recovery of kaempferol was 100.4% with the RSD of 1.49%. The content of quercetin in C. Heterophylla male flower was 0. 35 to 0. 58 mg/g and that of kaempferol ranged from 0. 174 to 0.512 mg/g. [ Conclusion] The method is simple and rapid with a good reproducibility,which can be used to determine the content of quercetin and kaempferol in C. Heterophylla male flower.关键词
高效液相色谱法/榛子雄花/槲皮素/山萘素/含量测定Key words
RP-HPLC/C. Heterophylla male flower/ Quercetin/ Kaempferol/ Content determination分类
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徐建,王婷婷,杨波,张显涛..HPLC法测定榛子雄花中槲皮素和山萘素的含量[J].安徽农业科学,2012,(5):2669-2670,2673,3.
