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蕨菜组织培养技术的研究

方利娟 苏仕林 蒲仕明

安徽农业科学Issue(6):3239-3240,3262,3.
安徽农业科学Issue(6):3239-3240,3262,3.

蕨菜组织培养技术的研究

Study on Tissue Culture Technology of Pteridium aquilinum L.kuhn

方利娟 1苏仕林 1蒲仕明1

作者信息

  • 1. 百色学院化学与生命科学系,广西百色533000
  • 折叠

摘要

Abstract

[Objective] To study the tissue culture technology of petiole from Pteridium aquilinum L.. [ Method ] By adding different concentration of 6-BA and IAA into the 1/2 MS medium, their effects on the tissue culture technology of Pteridium aquilinum L. Were studied. [ Result ] 1/2 MS +0.2 mg/L 6-BA +0.4 mg/L 1BA was the best callus induction medium with the induction rate of 82% ; 1/2 MS +0. 2 mg/L IBA +0. 2 mg/L 6-BA was the best callus proliferation medium with the proliferation multiple of 8.07; the best rooting media was 1/2 MS + 0.4 mg/L IBA with the rooting rate of 100% , the roots number was large,and the root was lony and robust. [Conclusion]The experiment provided references for the tissue culture of Pteridium aquilinum L..

关键词

蕨菜/叶柄/组织培养

Key words

Pteridium aquilinum L. /Petiole/Tissue culture

分类

生物科学

引用本文复制引用

方利娟,苏仕林,蒲仕明..蕨菜组织培养技术的研究[J].安徽农业科学,2012,(6):3239-3240,3262,3.

基金项目

广西教育厅科研项目(200807MS012). (200807MS012)

安徽农业科学

0517-6611

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