安徽农业科学Issue(6)：3243-3244,3250,3.
应用生物信息学分析大米过敏原RAG1的B细胞表位
Bioinformatics Analysis on B Cell Epitopes of Rice Allergen RAG1
摘要
Abstract
[Objective] To predict secondary structure and B cell epilopes of the rice major allergen RAG1. [ Method ] The amino acid sequence of rice allergen RAG1 was acquired from Expasy protein database. The secondary structure of RAG1 was predicted by DNAStar Protean software with Gamier-Robson program, Chou-Fasman program and Karplus-Schulz program; the B cell epitopes of RAG1 was predicted with the Kyte-Doolittle hydrophilic program, Emini surface accessibility program and Jameson-Wolf antigenic index program. [ Result] The predictions on secondary structure and B cell epitopes showed that the regions of 33 -44, 119 - 129, 155 - 163 were the dominant B cell epitopes. [Conclusion] This study predicted the potential dominant B cell epitopes in rice allergen RAG1 by comprehensive use of multi-methods and multi-parameters, and provided a theoretical basis for further researches on identification, antigen modification and epitope vaccine design of RAG1 B cell epitopes.关键词
大米过敏原/RAG1/二级结构/B细胞表住Key words
Rice allergen/ RAG1/ Secondary structure/ B cell epitopes分类
农业科技引用本文复制引用
李燕芳,何颖,邹泽红..应用生物信息学分析大米过敏原RAG1的B细胞表位[J].安徽农业科学,2012,(6):3243-3244,3250,3.基金项目
国家自然科学基金项目(30771240)和广州市教育系统科研创新学术团队(B94118)课题. （30771240）
