安徽农业科学Issue(6)：3253-3257,5.
新疆饲料乳酸菌的多样性与系统进化分析
Diversity and Phylogenetic Analysis of Lactic Acid in Forage of Xinjiang
摘要
Abstract
[Objective] The aim was to conduct preliminary investigations and diversity analysis of lactic acid bacteria resources in forage from Turpan of Xinjiang. [ Method ] The lactic acid bacteria in the three kinds of forage ingredients in Xinjiang were isolated by using plate separation method and screened by MRS + CaC03 solid medium. Morphological, physiological and biochemical identification and 16S rDNA gene sequence analysis were carried out to the isolated eighty strains of lactic acid bacteria, to explore its taxonomic status. [ Result ] Twenty strains of lactic acid bacteria were obtained from alfalfa, forty - one from wheat, and ninety from corn. The physiological and biochemical I-dentification and 16S rDNA gene sequence analysis results showed that the eighty strains of lactic acid bacteria belonged to two genera, namely , Laclobaeillus, Enterocoecus; 7 species, Lactobacillus casei, Lactobacillus rhamnosus, Lactobacillus paracasei, Entercoccus faecium, Enter-coccus durans, Laclobaeillus plantarum, Entercoccus hirae. Laclobaeillus casei and Entercoccus faecium were ubiquitous in the three kinds of forage ingredients. Besides these two lactic acid bacteria, there were Lactobacillus rhamnosus, Lactobacillus paracasei, Lactobacillus plantarum in wheat, Lactobacillus plantarum, Lactobacillus paracasei, Entercoccus hirae, Entercoccus durans in alfalfa, Lactobacillus plantarum, Entercoccus durans in corn. [Conclusion] There is a big diversity of lactic acid bacteria in different forage from Turpan of Xinjiang, in which Lactobacillus casei, Entercoccus faecium are the key bacteria for forage fermentation.关键词
饲料/乳酸菌/16S rDNA/多样性Key words
Forage/ Lactic acid bacteria/ 16S rDNA/ Diversity分类
农业科技引用本文复制引用
热娜·米吉提,古丽斯玛依·艾拜都拉,苏豫梅,周秀文,李越中,乌斯满·依米提..新疆饲料乳酸菌的多样性与系统进化分析[J].安徽农业科学,2012,(6):3253-3257,5.基金项目
新疆大学自然科学基金资助项目(070378) （070378）
山东大学微生物技术国家重点实验开放课题资助(M2011-07). （M2011-07）
