安徽农业科学Issue(6)：3382-3384,3564,4.
山苍子内生细菌的分离及抑菌活性研究
Isolation of Endophytic Bacteria from Litsea cubeba and Study of Their Antifungal Activity
摘要
Abstract
[Objective] To research the antifungal activity and biocontrol efficacy of endophytic bacteria from Litsea cubeba. [ Method] 52 bacteria strains were isolated from litsea cubeba,23 were from roots,8 from stems,7 from leaves and 14 from fruits. The antifungal activity to plant pathogen fungi of all these 52 strains were detected by antagonistic test. [Result] 5 strains were found to inhibit many pathogen fungi including Botryosphaeria dothidea;Rhixoctonia cerealis V. Hoeven;Botrytis cinerea;Fusarium graminearum;Colletotrichum gloeosporioides and Phytophthora capsici Leonian. Field trial was conducted in further,and the results showed one endophytic bacteria strain isolated from the leaf of Litsea cubeba controlled Phytophthora capsici Leonian well, promoted the growth of chili in addition, this strain was mostly closed to Bacillus megaterium based on 16S rDNA partial sequence identification (Genbank No. JN252095). [Conclusion] The results indicated that the endophytic bacteria of Litsea cubeba were useful potential antifungal resource in agriculture which needed to be further studied.关键词
山苍子/内生菌/对峙法/抑菌活性Key words
Litsea cubeba/Endophytic bacteria/Antagonistic tests/Antifungal activity分类
农业科技引用本文复制引用
林娜,游玲,郭华,魏琴..山苍子内生细菌的分离及抑菌活性研究[J].安徽农业科学,2012,(6):3382-3384,3564,4.基金项目
四川省教育厅青年基金项目(2009JY0149) （2009JY0149）
发酵资源与应用四川省高校重点实验室基金项目(2010 KFJ002) （2010 KFJ002）
宜宾市科技局重点项目(2010NY005). （2010NY005）
