安徽农业科学Issue(7)：3869-3871,3.
番茄组织培养与试管苗繁育
Inducing of Adventitious Buds from Tomato Callus and Rooting
摘要
Abstract
[Objective] The aim was to establish efficient regeneration system of tomato so as to prepare for transformation of tomato genetic. [Method] Cultivate tomato seeds processed aseptically to get plantlets. Then, cultivate leaves cut from plantlets in the MS with 3.0 mg/L6-BA + 0.3 mg/L IAA to induce callus. Finally, compare different hormones and concentration on induction of adventitious buds from tomato callus and rooting. [Result] The best medium was MS +2.0 mg/L 6-BA +0. 3 mg/L IAA +30 mg/L sugar,in which many embryo calli were produced; The best medium for rooting was 1/2MS + 1.0 mg/L IAA. [Conclusion] Appropriate choice of hormone concentrations is the key to establish efficient regeneration system for tomato.关键词
番茄/组织培养/愈伤组织/不定芽/生根Key words
Tomato/Tissue culture/Callus/Adventitious duds/Rooting分类
农业科技引用本文复制引用
张丽梅..番茄组织培养与试管苗繁育[J].安徽农业科学,2012,(7):3869-3871,3.
