安徽农业科学Issue(7)：3903-3905,3.
ramp在提高桂圆RAPD分析效率中的应用研究
Research on Application of Ramp to Improve the Efficiency of RAPD in Longan
梅志强 1李春红 2段承刚 1刘晓燕1
作者信息
- 1. 四川泸州医学院医学基础研究中心,四川泸州646000
- 2. 泸州医学院药物与功能性食品研究中心,四川泸州646000
- 折叠
摘要
Abstract
[Objective] This study aimed to detect a modified method for improving the efficiency of RAPD technology. [Method] Longans collected from Sichuan, Fujian, Guangxi, Guangdong and Hainan were used as experimental materials. Total genomic DNA was extracted from longan leaves with modified CTAB method. 18 random primers were selected for RAPD amplification with ramp rates of 0. 3 ℃/s and 3. 0 ℃/s, respectively. Effects of different ramp rates on RAPD amplification were compared and the relationship among longan samples was analyzed. [ Result] The resolution and yield could be improved significantly by declining the ramp rate from 3.0 °C/s to 0. 3 °C/s. Cluster analysis showed that similarity coefficient of the five varieties of longan ranged from 0. 69 to 0. 76. [ Conclusion] This study had confirmed that reducing ramp rate could significantly increase the resolution and yield of RAPD technology, which had provided a new method to improve the efficiency of RAPD.关键词
RAPD/ramp/桂圆/效率Key words
RAPD/ ramp/ Longan/ Efficiency分类
农业科技引用本文复制引用
梅志强,李春红,段承刚,刘晓燕..ramp在提高桂圆RAPD分析效率中的应用研究[J].安徽农业科学,2012,(7):3903-3905,3.
