安徽农业科学Issue(7)：3986-3988,3.
双酶水解制备大豆玉米活性多肽的研究
Preparation of Active Peptides from Corn and Soybean by Bi-enzyme Hydrolysis
摘要
Abstract
[Objective] The aim was to study the optimal test condition for preparation of active peptides from corn and soybean by bi-enzyme hydrolysis. [Method] Taking soybean peptide and corn peptide as materials, different concentrations of soy-corn protein isolate solution were prepared. Through pretreatment, and based on compound protease and flavor protease, neutral protease, papaya protease, pineapple hydrolysis , and by measuring the degree of hydrolysis activity and enzyme activity, tested enzyme was determined. Mixing two of those enzymes in certain proportion and by orthogonal test, the optimal condition of enzyme hydrolysis of a double was determined. [ Result] The optimal hydrolysis condition was 2% of substrate concentration, 1:1 of hydrolyze compound protease and flavor protease, and 8 h of hydrolysis time, 75. 14% of DH. [Conclusion] The study provided a basis for the development and application of active peptides from corn and soybean by bi-enzyme hydrolysis.关键词
大豆蛋白/玉米蛋白/双酶水解/多肽Key words
Soy protein/ Corn protein/ Bi-enzyme hydrolysis/ Peptides分类
农业科技引用本文复制引用
牟明峰,高云,宫关,孟建彪,张鹏..双酶水解制备大豆玉米活性多肽的研究[J].安徽农业科学,2012,(7):3986-3988,3.基金项目
辽宁省教育厅科学研究计划资助项目(L2010203) （L2010203）
辽宁省"十一五"教育科学规划立项课题(JG08 DB133) （JG08 DB133）
辽宁科技大学第七期大学生科研训练计划项目. （）
