安徽农业科学Issue(7)：4169-4172,4.
弱光胁迫对黑藻生理生化特性的影响
Effects of Light Intensity on Physiological and Biochemical Characteristics of Hydrilla verticillata(L.f.)Royle
摘要
Abstract
[Objective] The aim was to study the physiological reactions of H. Verticillata(L. F. ) Royle under different light intensity, to provide a theoretical basis for developing introduction and cultivation of submerged plant and optimization and allocation of initial community. [Method] H. Verticillata(L. F. ) Royle plants preprocessed were cultured under different light intensity(7 800, 3 100, 1 250, 500, 200 lx) respectively. The first sampling was the fifth after cultivation, and then sampling every 5 days, 6 times in total. The soluble protein content, malonaldehyde(MDA) content and superoxide dismutase(SOD) activity and peroxidase(POD) activity and catalase( CAT) activity were determined. [ Result] The results showed that H. Verticillata( L. F. ) Royle had the abilities of decontaminating and the adaptation to weak light, but the tolerance decreased with prolongation of exposure time. SOD activity increased twenty days later at the light intensity of 1 250 lx. The activity of CAT and POD began to increase while the soluble protein began to decrease at 500 lx light intensity. The SOD activity reached the peak when using 200 lx light intensity cultivating 5 days, then continue decreased, while the CAT activity continued to fluctuate greatly, showing the activated oxygen metabolism of H. Verticillata(L. F. ) Royle in vivo was imbalanced under 200 lx light intensity, and the cells might suffer irreversible damage, so 200 lx might be the light compensation point of H. Verticllata( L. F. ) Royle. MDA content did not change significantly during the whole test, and the difference among different treatments was not significant. [ Conclusion] Weak light stress can cause generation and accumulation of ROS in H. Verticillata(L. F. ) Royle, inducing the antioxidant enzymes activity raising. When the stress exceeds a certain strength, the antioxidant enzyme activity is inhibited, reactive oxygen species can not be cleared, then forms oxidative damage to plant body, which may be one of the important mechanisms of affecting submerged plant restoration in the eutrophication water body.关键词
黑藻/弱光/抗氧化性保护酶/生态修复Key words
H. Verticillata(L. F. ) Royle/ Low light/ Anti-oxidation enzymes/ Ecological restoration分类
农业科技引用本文复制引用
薛维纳,彭岩波,宋祥甫,邹国燕..弱光胁迫对黑藻生理生化特性的影响[J].安徽农业科学,2012,(7):4169-4172,4.基金项目
山东省高等学校优秀青年教师国内访问学者项目. （）
