摘要
Abstract
To investigate the effect of ketamine on the capabilities of memory, content of and learning and AchE, ChAT of hippocampus in neonatal rats. Methods 80 SD rats, aged 7 days were randomly divided into group C, Kl, K2, K3 ,20 rats in each group. Group C was treated with 2 ml physiological saline; group Kl was treated with ketamine 80 mg/kg once; group K2 was treated with ketamine gomgllcg once a day for 2 days; group K3 was treated with ketamine gomgllcg once a day for 3 days. 10 rats were killed each group the next day after the last injection. After testing the behavior at the 21st day by Morris water maze( MWM ), the rest of rats were killed and isolated the hippocampus for determination of ChAT and AchE. Results MWM: There was no significant difference between group C and group Kl, group K2 and group K3, the latency period way longer and the frequency to cross the plat way less in group K2,K3 than that in group C,Kl( P <0. 05 ). The results of enzymes:8 days age, there were significant difference ( P <0. 05 );27 days age, there was no significant difference between group C and group Kl, group K2 and group K3. There was significant difference between group Kl, C and group K2, K3 ( P < 0. 05 ). Conclusion Ketamine can decrease the spatial learning and memory behavior of neonatal rats, and it may be related to the cholinergic nervous system of hippocampus; single done causes short-term change, and repeated medicine can lead to long-time influence .关键词
氯胺酮/海马/乙酰胆碱酯酶/乙酰胆碱转移酶/Morris水迷宫Key words
ketamine/ hippocampus/ acetylcholinesterase/ choline acetyltransferase/ Morris water maze分类
医药卫生
