安徽医科大学学报2012，Vol.47Issue(2)：137-141,5.
miR-92a反义寡核苷酸对胃癌细胞增殖和凋亡的影响
Effect of anti-miR-92a oligonucleotides on proliferation and apoptosis of gastric cancer cell lines
摘要
Abstract
To study the effect of anti-miR-92a oligonucleotides on proliferation and apoptosis of gastric cancer cell lines. Methods Gastric cancer cell lines, SGC-7901 and MKN-45 ,were cultured in vitro and transfect-ed with anti-miR-92a oligonucleotides. Expression of miR-92a in the cells was measured by quantitative real-time PCR( qRT-PCR ). MTT assay was used to evaluate the cell proliferation. Cell apoptosis was detected by flow cytome-try ( AnnexinV-FITC/PI assay ) and Hoechst 33258 staining. Results Expression of miR-92a in two cell lines was significantly down-regulated by the transfection of anti-miR-92a oligonucleotides for 48 h. At the same time, the cell proliferation was inhibited and apoptosis was increased remarkably by the transfection. Further, microscopic observation with Hoechst 33258 staining showed apoptotic feature of cell nucleus change in the transfected gastric cancer cell lines while there was no change in the control group. Conclusion Targeted inhibition of miR-92a with antisense oligoribonucleotide effectively suppresses gastric cancer cell growth by inducing apoptosis. miR-92a may be a potential target for gastric cancer therapy.关键词
胃肿瘤/微小RNA/反义寡核苷酸/增殖/凋亡Key words
stomach neoplasms/ microRNAs/ antisense oligonucleotides/ proliferation/ apoptosis分类
医药卫生引用本文复制引用
仲飞,郝吉庆,王芳,马泰,范璐璐,孙国平..miR-92a反义寡核苷酸对胃癌细胞增殖和凋亡的影响[J].安徽医科大学学报,2012,47(2):137-141,5.基金项目
教育部高校博士学科点专项科研基金(编号:20100171120090) （编号:20100171120090）
广东省医学科研基金(编号:B2009079) （编号:B2009079）
