安徽医科大学学报2012，Vol.47Issue(3)：241-244,4.
棕榈酸致大鼠L6肌细胞胰岛素抵抗与JNK1活化的关系
Association of JNK1 activation with palmitic acid-induced insulin resistance in L6 rat muscle cells
摘要
Abstract
To establish palmitic acid( PA ) induced insulin resistance ( IR ) model in rat L6 muscle cells, and initially explore its mechanism and the association with the activation of JNK1. Methods Rat L6 skeletal muscle cells was cultured by different concentration of PA for 2, 4, 8, 12, 24 and 36 h respectively. The concentration of remained glucose in each group was measured by Glucose Test Kit respectively, and observed the effects of different concentration of PA on glucose uptake in L6 skeletal muscle cells. The effects of PA to L6 skeletal muscle cells activity were measured by MTT. Protein expression of JNK1 and p-JNKl in normal group and in IR group with PA-induced were measured by Western blot. Results When incubated in 0. 4 mmol/L PA for 12 ~ 36 h, or in 0. 6 and 0. 8 mmol/L PA for 8 ~ 24 h, the concentration of glucose remained in PA group was significantly higher than that in normal group( P <0. 05 ). MTT results suggested that incubated in 0. 8,1. 0 mmol/L PA for 24 ~ 36 h had effected on cells activity. The protein expression of JNK1 and p-JNKl in NG group was low, while it was higher in IR group, especially for p-JNKl. But compared with normal group, the expression of JNK1 in IR group had no statistical significance, the expression of p-JNKl in IR group was significantly higher( P < 0. 05 ). Conclusion A certain concentration of PA may lead to IR in rat L6 muscle cells, which may be associated with JNK1 activation. Inhibition of excessive activation of JNK1 may be a new target for treatment of IR.关键词
L6肌细胞/胰岛素抵抗/JNK/棕榈酸Key words
L6 muscle cells/ insulin resistance/ JNK/ palmitic acid分类
医药卫生引用本文复制引用
杨宁宁,王佑民,陈冬,陈明卫..棕榈酸致大鼠L6肌细胞胰岛素抵抗与JNK1活化的关系[J].安徽医科大学学报,2012,47(3):241-244,4.基金项目
安徽高校省级自然科学研究重点项目(编号:KJ2011 A161) （编号:KJ2011 A161）
