安徽医科大学学报2012，Vol.47Issue(3)：270-273,4.
IL-8和Eotaxin在哮喘大鼠中的表达及地塞米松的作用
Expression of IL-8 and Eotaxin in rat asthma modeland role of dexmethasone
摘要
Abstract
investigate the expression of IL-8 and Eotaxin in peripheral blood, bronchoalveolar lav-age fluid ( BALF ), mRNA and protein expressions of Eotaxin in lung tissue of asthma rat, and observe the effection of IL-8 and Eotaxin on dexamethasone( DXM ). Methods Thirty SD rats were randomly divided into control group, asthma model group and DXM group. Asthma rat model was established by sensitization and stimulation with ovalbumin( OVA ). Cell differential counting was conducted in BALF. IL-8 and Eotaxin levels in serum and BALF were detected by enzyme linked immunosorbent assay( ELISA ). The lung tissue slices were stained with HE. The mRNA and protein expressions of Eotaxin in lung tissue were measured with real-time fluorescent quantitative PCR and immunohistochemical techniques. Results Total white blood cell number, the percentage of eosinophils, neu-trophils and lymphocytes in BALF of asthma group were significantly higher than those in control group( P < 0. 01 ); The expression of IL-8, Eotaxin in serum and BLAF of asthma group were increased than those in control group( P <0. 01 ),which was descreased in dexamethasone treated group than those in asthma group( P <0. 05 ). The mRNA and protein expressions of Eotaxin in lung tissue of asthma group were higher than those both in control group and asthma group( P <0. 05 ,P < 0. 01 ). Conclusions IL-8 and Eotaxin are involved in inflammation of asthma, and dexmethasone may inhibit the expression of IL-8 and Eotaxin to relieve aggregation and activation of neutrophil and eosinophil. Which may be one mechanism of dexmethasone to attenuate inflammation of asthma.关键词
哮喘/趋化因子/白细胞介素-8/嗜酸性粒细胞趋化因子Key words
asthma/ chemokine/ interleukin-8/ Eotaxin分类
医药卫生引用本文复制引用
汪浩,武晓兰,谭红霞,范晓云..IL-8和Eotaxin在哮喘大鼠中的表达及地塞米松的作用[J].安徽医科大学学报,2012,47(3):270-273,4.基金项目
国家自然科学基金项目(编号:81100027) （编号:81100027）
安徽省高校省级自然科学研究项目(编号:KJ2011A176) （编号:KJ2011A176）
院国家青年基金培育计划(编号:2010KJ13) （编号:2010KJ13）
安徽省教育厅教学研究项目(编号:20100350) （编号:20100350）
